High-resolution Autoradiography

The Spatial Resolution of Silicon-Based Electron Detectors in β- Autoradiography

Thin tissue autoradiography is an imaging modality where ex-vivo tissue sections are placed in direct contact with autoradiographic film. These tissue sections contain a radiolabelled ligand bound to a specific biomolecule under study. This radioligand emits βor β+ particles ionising silver halide crystals in the film. High spatial resolution autoradiograms are obtained using low energy radiois...

High-resolution autoradiography of drugs in fixed tissues.

High-resolution Mapping of Satellite Using Biotin-labeled DNA Probes DNA

A8STRACT We have developed a novel method for high resolution mapping of specific DN,5 sequences after in situ hybridization. DNA probes, labeled with biotin-nucleotides in conventional nick-translation reactions, are hybridized to cytological preparations and detected with affinity-purified rabbit antibiotin antibodies followed by antibodies to rabbit IgG that are conjugated to fluorescent or ...

Skin research and drug localization with receptor microscopic autoradiography.

For the localization of drugs and related functional characterization, cellular-subcellular resolution can be achieved with radiolabelled compounds of high specific activity and receptor microscopic autoradiography, which is the method of choice for high-resolution qualitative and quantitative imaging. Detailed information together with integrative surveys can be obtained which is impossible wi...

Tritium and Phosphorus-32 in High-Resolution Autoradiography.

The sensitivity of monolayers of Ilford L-4 nuclear emulsion to beta(-)-particles is approximately 0.20 grain per particle emitted for H(3), and 0.025 grain per particle for p(32). The sensitivity for thick layers is 1.3 grains per tritium beta(-)-particle emitted within the emulsion. In electron microscopic autoradiographs the maximum resolution is approximately 0.1 micron for H(3) and 0.3 mic...